Gamma Enolase Antibody / NSE / ENO2
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P09104
Host
Mouse
Reactivity
Human
Immunogen
A recombinant fragment of human NSE gamma (within amino acids 416-433) was used as the immunogen for the Gamma Enolase antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
ENO2/4507
Applications
WB, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the Gamma Enolase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This Gamma Enolase antibody is available for research use only.
Storage Conditions
Aliquot the Gamma Enolase antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the Gamma Enolase antibody should be determined by the researcher.
Location
Cytoplasm
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