Recombinant CD27 Antibody

Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL

UniProt

P26842

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human full-length protein was used as the immunogen for the recombinant CD27 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

LPFS2/8574R

Type

Recombinant

Applications

IHC-P, WB

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the recombinant CD27 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This recombinant CD27 antibody is available for research use only.

Storage Conditions

Aliquot the recombinant CD27 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the recombinant CD27 antibody should be determined by the researcher.

Location

Cell Surface

Image Legend

Western blot testing of human Raji and Ramos cell lysate with recombinant CD27 antibody. Predicted molecular weight ~29 kDa but may be observed at higher molecular weights due to glycosylation.