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CD57 Antibody / HNK-1

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q9P2W7

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein (within amino acids 1-200) from the human protein was used as the immunogen for the CD57 antibody.

Clonality

Monoclonal

Isotype

IgG2 λ

Clone

NK1/7565

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CD57 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CD57 antibody is available for research use only.

Storage Conditions

Aliquot the CD57 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CD57 antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic

Image Legend

IHC staining of FFPE human stomach tissue with CD57 antibody (clone NK1/7565) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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