CXCR5 Antibody / CD185 / BLR1 / MDR15

Burkitt s lymphoma receptor 1 (Blr1), also called CD185, CXCR5 and MDR15, is a lymphocyte specific chemokine receptor expressed at low levels in secondary lymphoid tissues and in defined structures of the cerebellum. The G protein-coupled receptor has significant homology to other chemokine receptors. Stimulation of Blr1 by its ligand, B-lymphocyte chemoattractant (BLC) results in an influx of calcium into the cell and the chemotaxis of the cell. Blr1 is required for B-cell migration into splenic and Peyer s patch follicles. BLC expression in Peyer s patches is highest in germinal centers, where B cells undergo somatic mutation and affinity maturation.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P32302

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the CXCR5 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

CXCR5/8146R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the CXCR5 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This CXCR5 antibody is available for research use only.

Storage Conditions

Aliquot the CXCR5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the CXCR5 antibody should be determined by the researcher.

Location

Cell membrane

Image Legend

IHC staining of FFPE human tonsil tissue with CXCR5 antibody (clone CXCR5/8146R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.