CD3 epsilon Antibody
Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR) . Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.
Product Specifications
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P07766
Host
Mouse
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the CD3 epsilon antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RC3e/6966
Type
Recombinant
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the CD3 epsilon antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This CD3 epsilon antibody is available for research use only.
Storage Conditions
Aliquot the CD3 epsilon antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.nsjbio.com/tds-pdf/cd3-epsilon-antibody-rc3e6966-v4208
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD3 epsilon antibody should be determined by the researcher.
CAS Number
9000-83-3
Location
Cell surface, Cytoplasm
Image Legend
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