DRAP1 Antibody

NC2 (negative cofactor 2) is a dimeric histone-fold complex that represses RNA polymerase II transcription through binding to TBP and inhibiting the transcription factors TFIIA and TFIIB. NC2 consists of two subunits, termed NC2a and NC2beta, and these subunits dimerize and bind to TBP-promoter complexes via histone fold domains of the H2A-H2B type. NC2 associates with promoters in a manner that correlates with transcriptional activity and with occupancy by basal transcription factors. NC2 binds directly to DNA, and the binding of NC2 to TBP-promoter complexes affects the conformation of DNA, and results in the inhibition of TFIIB.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Q14919

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human DRAP1 protein was used as the immunogen for the DRAP1 antibody.

Clonality

Monoclonal

Isotype

IgG2b

Clone

PCRP-DRAP1-1A8

Applications

FACS, IF, IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the DRAP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This DRAP1 antibody is available for research use only.

Storage Conditions

Aliquot the DRAP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the DRAP1 antibody should be determined by the researcher.

Location

Nucleus, Cytoplasm

Image Legend

Immunofluorescent staining of PFA-fixed human HeLa cells with DRAP1 antibody (clone PCRP-DRAP1-1A8) followed by goat anti-mouse IgG-CF488 (green) ; Red = CF640R phalloidin.