DRAP1 Antibody
NC2 (negative cofactor 2) is a dimeric histone-fold complex that represses RNA polymerase II transcription through binding to TBP and inhibiting the transcription factors TFIIA and TFIIB. NC2 consists of two subunits, termed NC2a and NC2beta, and these subunits dimerize and bind to TBP-promoter complexes via histone fold domains of the H2A-H2B type. NC2 associates with promoters in a manner that correlates with transcriptional activity and with occupancy by basal transcription factors. NC2 binds directly to DNA, and the binding of NC2 to TBP-promoter complexes affects the conformation of DNA, and results in the inhibition of TFIIB.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
Q14919
Host
Mouse
Reactivity
Human
Immunogen
Recombinant human DRAP1 protein was used as the immunogen for the DRAP1 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
PCRP-DRAP1-1A8
Applications
FACS, IF, IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the DRAP1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This DRAP1 antibody is available for research use only.
Storage Conditions
Aliquot the DRAP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the DRAP1 antibody should be determined by the researcher.
Location
Nucleus, Cytoplasm
Image Legend
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