MyoD1 Antibody
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this mAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P15172
Host
Mouse
Reactivity
Human
Immunogen
Recombinant mouse MyoD1 protein was used as the immunogen for the MyoD1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
MYOD1/7149
Applications
IHC-P
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the MyoD1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MyoD1 antibody is available for research use only.
Storage Conditions
Aliquot the MyoD1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the MyoD1 antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Frequently Asked Questions
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