MGMT Antibody
Cancer chemotherapeutic alkylating agents (e.g. BCNU, ) act by inducing formation of lethal cross-links at the O6- alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6- position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P16455
Host
Rabbit
Reactivity
Human
Immunogen
A recombinant partial protein (within amino acids 1-100) from the human protein was used as the immunogen for the MGMT antibody.
Clonality
Monoclonal
Isotype
IgG κ
Clone
MGMT/8364R
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MGMT antibody is available for research use only.
Storage Conditions
Aliquot the MGMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the MGMT antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Frequently Asked Questions
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