MGMT Antibody
Cancer chemotherapeutic alkylating agents (e.g. BCNU, ) act by inducing formation of lethal cross-links at the O6- alkylguanine position in DNA. MGMT transfers alkyl adducts from the O6- position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely to be drug resistant.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P16455
Host
Rabbit
Reactivity
Human
Immunogen
Purified His-tagged MGMT protein was used as the immunogen for the MGMT antibody.
Clonality
Monoclonal
Isotype
IgG κ
Clone
MGMT/8319R
Applications
IHC-P, WB
Purity
Protein A/G affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Aliquot the MGMT antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
Limitations
This MGMT antibody is available for research use only.
Storage Conditions
Aliquot the MGMT antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the MGMT antibody should be determined by the researcher.
Location
Nucleus
Image Legend
Western blot testing of human MCF7 cell lysate with MGMT antibody. Predicted molecular weight: ~23 kDa.
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