PTEN Antibody

PTEN (phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase) contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. It was identified as a tumor suppressor that is mutated in a large number of cancers, including sporadic brain, breast, kidney, and prostate cancers.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P60484

Host

Mouse

Reactivity

Human

Immunogen

Recombinant full-length human PTEN protein was used as the immunogen for the PTEN antibody.

Clonality

Monoclonal

Isotype

IgG κ

Clone

PTEN/7344

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the PTEN antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This PTEN antibody is available for research use only.

Storage Conditions

Aliquot the PTEN antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the PTEN antibody should be determined by the researcher.

Location

Cytoplasm, Nucleus

Image Legend

IHC staining of FFPE human prostate carcinoma tissue with PTEN antibody (clone PTEN/7344) at 2ug/ml. Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.