Pulmonary Surfactant-Associated Protein D Antibody

Pulmonary surfactant is primarily responsible for lowering the surface tension at the air-liquid interface in the alveoli, a process that is essential for normal respiration. Pulmonary surfactant is a mixture of phospholipids and proteins, including four distinct surfactant-associated proteins (SPs), SP-A, SP-B, SP-C, SP-D. SP-B and SP-C are predominantly hydrophobic proteins that associate with lipids to promote the absorption of surfactant phospholipids and to reduce the surface tension in the alveoli. SP-A and SP-D are large multimeric proteins belonging to the family of calcium-dependent lectins, designated Collectins, which contribute to the innate immune system. Both SP-A and SP-D have been shown to protect against microbial challenge through binding to the lipid components of the bacterial cell wall and facilitating the rapid removal of microbials.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P35247

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein (within amino acids 241-336) from the human protein was used as the immunogen for the Pulmonary Surfactant-Associated Protein D antibody.

Clonality

Monoclonal

Isotype

IgG2b λ

Clone

SFTPD/4362

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the Pulmonary Surfactant-Associated Protein D antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This Pulmonary Surfactant-Associated Protein D antibody is available for research use only.

Storage Conditions

Aliquot the Pulmonary Surfactant-Associated Protein D antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Pulmonary Surfactant-Associated Protein D antibody should be determined by the researcher.

Location

Secreted

Image Legend

Analysis of a HuProt (TM) microarray containing more than 19,000 full-length human proteins using Pulmonary Surfactant-Associated Protein D antibody (clone SFTPD/4362) . Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a mAb to its intended target. A mAb is considered to specific to its intended target, if the mAb has an S-score of at least 2.5. For example, if a mAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that mAb to protein X is equal to 29.