C-RET Antibody

The Ret proto-oncogene is structurally related to the growing family of tyrosine kinase transmembrane receptors and is involved in GDNF signaling. RET expression is reported in several regions of the central nervous system; in the developing cranial nerve ganglia and a subset of cells within dorsal root ganglia, in motor neurons in the spinal cord and hindbrain, in neuro-retina and the growing tips of the renal collecting ducts in developing kidney. Alterations in RET gene are associated with diseases including papillary thyroid carcinoma, multiple endocrine neoplasia (type 2A and 2B), familial medullary thyroid carcinoma, and a congenital developmental disorder known as Hirschsprung s disease.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P07949

Host

Mouse

Reactivity

Human

Immunogen

A recombinant partial protein (within amino acids 702-848) from the human protein was used as the immunogen for the c-RET antibody.

Clonality

Monoclonal

Isotype

IgG2c κ

Clone

RET/8789

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the c-RET antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This c-RET antibody is available for research use only.

Storage Conditions

Aliquot the c-RET antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the c-RET antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear, cell surface

Image Legend

IHC staining of FFPE human parathyroid tissue with c-RET antibody (clone RET/8789) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.