GATA-3 Antibody

GATA-3 (GATA binding protein 3) is a member of the GATA family of transcription factors. This 50kDa nuclear protein regulates the development and subsequent maintenance of a variety of human tissues, including hematopoietic cells, skin, kidney, mammary gland, and the central nervous system. Among several other roles, GATA-3 involved in luminal cell differentiation in the mammary gland and appears to control a set of genes involved in the differentiation and proliferation of breast cancer. The expression of GATA-3 is associated with the expression of estrogen receptor-alpha (ER) in breast cancer. GATA-3 has been shown to be a novel marker for bladder cancer. The study demonstrated that GATA-3 stained 67% of urothelial Carcinomas, but none of prostate or renal carcinomas.

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

P23771

Host

Rabbit

Reactivity

Human

Immunogen

A recombinant partial protein (within amino acids 150-250) from the human protein was used as the immunogen for the GATA-3 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

GATA3/7686R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Aliquot the GATA-3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This GATA-3 antibody is available for research use only.

Storage Conditions

Aliquot the GATA-3 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the GATA-3 antibody should be determined by the researcher.

Location

Nucleus

Image Legend

IHC staining of FFPE human ovarian cancer tissue with GATA-3 antibody (clone GATA3/7686R) . Inset: PBS used in place of primary Ab (secondary Ab negative control) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.