TAG-72 Antibody

Recognizes an oncofetal antigen of 220kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This mAb defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80% sensitivity and 93% specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of anti-TAG-72 and anti-GCDFP-15 is valuable in the diagnosis of apocrine carcinoma

Product Specifications

CAS Number

9000-83-3

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

UniProt

Not Known

Host

Rabbit

Reactivity

Human

Immunogen

Purified TAG-72 protein was used as the immunogen for the TAG-72 antibody.

Clonality

Recombinant Monoclonal

Isotype

IgG κ

Clone

TAG72/8317R

Type

Recombinant

Applications

IHC-P

Purity

Protein A/G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Aliquot the TAG-72 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Limitations

This TAG-72 antibody is available for research use only.

Storage Conditions

Aliquot the TAG-72 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the TAG-72 antibody should be determined by the researcher.

Location

Cytoplasm, Cell surface

Image Legend

IHC staining of FFPE human colon carcinoma tissue with TAG-72 antibody (clone TAG72/8317R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.