Anti-cdc34 Antibody

Mouse Monoclonal Antibody specific to cdc34

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.3.2.23,

Gene Name

CDC34

NCBI Gene ID

UniProt

P49427

Cellular Locus

Cytoplasm. Nucleus. Note=The phosphorylation of the C-terminal tail plays an important role in mediating nuclear localization. Colocalizes with beta-tubulin on mitotic spindles in anaphase.

Host

Mouse

Reactivity

Human

Immunogen

N-terminus 6x-His tagged fusion protein encoding full-length human Cdc34 expressed in E. coli.

Target Antigen

Ubiquitin-conjugating enzyme E2 R1

Target

cdc34

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF, IHC-P, IP

Field of Research

Neuroscience

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination (PubMed:22496338). Cooperates with the E2 UBCH5C and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Performs ubiquitin chain elongation building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. UBE2D3 acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Cooperates with the SCF(SKP2) E3 ligase complex to regulate cell proliferation through ubiquitination and degradation of MYBL2 and KIP1. Involved in ubiquitin conjugation and degradation of CREM isoform ICERIIgamma and ATF15 resulting in abrogation of ICERIIgamma- and ATF5-mediated repression of cAMP-induced transcription during both meiotic and mitotic cell cycles. Involved in the regulation of the cell cycle G2/M phase through its targeting of the WEE1 kinase for ubiquitination and degradation. Also involved in the degradation of beta-catenin. Is target of human herpes virus 1 protein ICP0, leading to ICP0-dependent dynamic interaction with proteasomes (PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421). {PubMed:10329681, PubMed:10373550, PubMed:10871850, PubMed:11675391, PubMed:12037680, PubMed:15652359, PubMed:17461777, PubMed:17698585, PubMed:19112177, PubMed:19126550, PubMed:19945379, PubMed:20061386, PubMed:20347421, PubMed:22496338}.

Additionnal Information

Immunoblotting and Immuno-precipitation: use at 1-5 ug/mL. <br><br>Positive controls: 293 cells and recombinant fusion protein.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human Cdc34.

Formulation

10 mM PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Cdc 34 is a ubiquitin conjugating enzyme that is essential for the transition from G1 to S phase in Saccharomyces cerevisiae. Human Cdc34 fully complements the budding yeast Cdc34 temperature sensitive mutant phenotype. In extracts of Xenopus laevis eggs, Cdc34 is required for the onset of DNA replication and appears to function in a multi-protein complex. Cdc34 is highly conserved among vertebrates and probably functions similarly in all eukaryotes to regulate the start of DNA synthesis.