Biotinylated Human GDF-15 / MIC-1 Protein, His, Avitag™

Growth Differentiation Factor 15 (GDF-15), also called Macrophage Inhibitory Cytokine 1 (MIC-1) . Expression of MIC-1 mRNA in monocytoid cells is up-regulated by a variety of stimuli associated with activation, including interleukin 1β, tumor necrosis factor α (TNF-α), interleukin 2, and macrophage colony-stimulating factor but not interferon γ, or lipopolysaccharide (LPS) . It is highly expressed in cardiomyocytes, adipocytes, macrophages, endothelial cells, and vascular smooth muscle cells in normal and pathological condition. GDF-15 increases during tissue injury and inflammatory states and is associated with cardiometabolic risk. Increased GDF-15 levels are associated with cardiovascular diseases such as hypertrophy, heart failure, atherosclerosis, endothelial dysfunction, obesity, insulin resistance, diabetes, and chronic kidney diseases in diabetes. Increased GDF-15 level is linked with the progression and prognosis of the disease condition.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 15.8 kDa. The protein migrates as 16 -17 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Accession Number

Q99988-1

Expression Region

Ala 197 - Ile 308

Host

E. coli

Target

GDF-15

Conjugation

Biotin-labeled

Tag

N-10xHis & N-Avi

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Biotinylated Human GDF-15 Protein, His,Avitag (Cat. No. GD5-H81Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human GFR alpha-like, Fc Tag (Cat. No. GFE-H5259) with a linear range of 0.2-13 ng/mL (QC tested).