TRMT61B Antibody / mRNA methyladenosine-N (1) -methyltransferase

Enables mRNA (adenine-N1-) -methyltransferase activity; rRNA (adenine) methyltransferase activity; and tRNA (adenine-N1-) -methyltransferase activity. Involved in mRNA methylation; mitochondrial tRNA methylation; and protein homooligomerization. Located in mitochondrion.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9BVS5

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids R7-N477) was used as the immunogen for the TRMT61B antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the TRMT61B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TRMT61B antibody is available for research use only.

Storage Conditions

After reconstitution, the TRMT61B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TRMT61B antibody should be determined by the researcher.

Location

Cytoplasmic (mitochondria)

Image Legend

Immunofluorescent staining of FFPE human intestinal cancer tissue with TRMT61B antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH8 EDTA buffer for 20 min.