UBE2O Antibody / Ubiquitin-conjugating enzyme E2 O

Ubiquitin-conjugating enzyme E2 O is a protein that in humans is encoded by the UBE2O gene. Enables ubiquitin conjugating enzyme activity and ubiquitin protein ligase activity. Involved in positive regulation of BMP signaling pathway; protein ubiquitination; and retrograde transport, endosome to Golgi. Located in cytoplasm and nuclear body.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9C0C9

Host

Rabbit

Reactivity

Human, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids R150-A1283) was used as the immunogen for the UBE2O antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the UBE2O antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This UBE2O antibody is available for research use only.

Storage Conditions

After reconstitution, the UBE2O antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the UBE2O antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human Daudi cells with UBE2O antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= UBE2O antibody.