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THEMIS Antibody

This gene encodes 'Thymocyte-expressed molecule involved in selection' a protein that plays a regulatory role in both positive and negative T-cell selection during late thymocyte development. The protein functions through T-cell antigen receptor signaling, and is necessary for proper lineage commitment and maturation of T-cells. Alternative splicing results in multiple transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q8N1K5

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids Q74-H588) was used as the immunogen for the THEMIS antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the THEMIS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This THEMIS antibody is available for research use only.

Storage Conditions

After reconstitution, the THEMIS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the THEMIS antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human JK cells with THEMIS antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= THEMIS antibody.

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