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UBA5 Antibody / UBE1DC1

Ubiquitin-like modifier-activating enzyme 5 (UBA5), also called Ubiquitin-activating enzyme E1 domain-containing protein 1 (UBE1DC1), is a protein that in humans is encoded by the UBA5 gene. This gene encodes a member of the E1-like ubiquitin-activating enzyme family. This protein activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. Alternative splicing results in multiple transcript variants. A pseudogene of this gene has been identified on chromosome 1.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9GZZ9

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids R37-D389) was used as the immunogen for the UBA5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the UBA5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This UBA5 antibody is available for research use only.

Storage Conditions

After reconstitution, the UBA5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the UBA5 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human colorectal adenocarcinoma tissue with UBA5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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