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Plagl1 Antibody / Lot1 / Mac

Zinc finger protein PLAGL1 (Pleiomorphic adenoma-like protein 1), also called Tumor suppressor ZAC and LOT 1 (Lost on transformation 1) is a protein that in humans is encoded by the PLAGL1 gene. Predicted to enable DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Acts upstream of or within regulation of gene expression and skeletal muscle cell differentiation. Predicted to be located in Golgi apparatus and nuclear body. Is expressed in several structures, including alimentary system; brain; embryo mesenchyme; heart; and sensory organ. Orthologous to human PLAGL1 (PLAG1 like zinc finger 1) .

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

B7ZP38

Host

Rabbit

Reactivity

Mouse

Immunogen

E. coli-derived recombinant mouse protein (amino acids M1-Q527) was used as the immunogen for the Plagl1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Plagl1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Plagl1 antibody is available for research use only.

Storage Conditions

After reconstitution, the Plagl1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Plagl1 antibody should be determined by the researcher.

Location

Nuclear, possibly cytoplasmic (ER)

Image Legend

Immunofluorescent staining of FFPE mouse RM1 cells with Plagl1 antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.

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