TDG Antibody / Thymine-DNA glycosylase

G/T mismatch-specific thymine DNA glycosylase is an enzyme that in humans is encoded by the TDG gene. The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL, Immunofluorescence: 5 µg/mL

UniProt

Q13569

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids Q15-R370) was used as the immunogen for the TDG antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the TDG antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TDG antibody is available for research use only.

Storage Conditions

After reconstitution, the TDG antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TDG antibody should be determined by the researcher.

Image Legend

Immunofluorescent staining of FFPE human HeLa cells with TDG antibody (green) and Alpha Tubulin mAb (red) . HIER: steam section in pH6 citrate buffer for 20 min.