Thrombospondin-3 Antibody / THBS3 / TSP3

Thrombospondin-3 (TSP3) is a protein that in humans is encoded by the THBS3 gene. The protein encoded by this gene belongs to the thrombospondin family. Thrombospondin family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentameric molecule linked by a single disulfide bond. This gene shares a common promoter with metaxin 1. Alternate splicing results in coding and non-coding transcript variants.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P49746

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids Q23-T486) was used as the immunogen for the Thrombospondin-3 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Thrombospondin-3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Thrombospondin-3 antibody is available for research use only.

Storage Conditions

After reconstitution, the Thrombospondin-3 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Thrombospondin-3 antibody should be determined by the researcher.

Location

Perinuclear, extracellular

Image Legend

IHC staining of FFPE human breast cancer tissue with Thrombospondin-3 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.