SerRS Antibody / Seryl-tRNA synthetase / SARS2

Seryl-tRNA synthetase, mitochondrial is an enzyme that in humans is encoded by the SARS2 gene. This gene encodes the mitochondrial seryl-tRNA synthethase precursor, a member of the class II tRNA synthetase family. The mature enzyme catalyzes the ligation of Serine to tRNA (Ser) and participates in the biosynthesis of selenocysteinyl-tRNA (sec) in mitochondria. The enzyme contains an N-terminal tRNA binding domain and a core catalytic domain. It functions in a homodimeric form, which is stabilized by tRNA binding. This gene is regulated by a bidirectional promoter that also controls the expression of mitochondrial ribosomal protein S12. Both genes are within the critical interval for the autosomal dominant deafness locus DFNA4 and might be linked to this disease. Multiple transcript variants encoding different isoforms have been identified for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9NP81

Host

Rabbit

Reactivity

Human

Immunogen

E. coli-derived recombinant human protein (amino acids E46-S518) was used as the immunogen for the SerRS antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the SerRS antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This SerRS antibody is available for research use only.

Storage Conditions

After reconstitution, the SerRS antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the SerRS antibody should be determined by the researcher.

Location

Cytoplasmic (mitochondria)

Image Legend

Immunofluorescent staining of FFPE human A549 cells with SerRS antibody (red) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.