VAPA Antibody / VAP33

VAMP-Associated Protein A (Vesicle-Associated Membrane Protein-Associated Protein A), also called 33 kDa VAMP-associated protein (VAP33) is a protein that in humans is encoded by the VAPA gene. The protein encoded by this gene is a type IV membrane protein. It is present in the plasma membrane and intracellular vesicles. It may also be associated with the cytoskeleton. This protein may function in vesicle trafficking, membrane fusion, protein complex assembly and cell motility. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q9P0L0

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids KHEQILVLDPPTDLKFK from the human protein were used as the immunogen for the VAPA antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the VAPA antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This VAPA antibody is available for research use only.

Storage Conditions

After reconstitution, the VAPA antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the VAPA antibody should be determined by the researcher.

Location

Cell surface, cytoplasmic, nuclear membrane

Image Legend

IHC staining of FFPE human cervical cancer tissue with VAPA antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.