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TRAM1 Antibody / TRAM

Sequence analysis predicted that human TRAM1 (Translocation-Associating Membrane Protein 1) is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. Functional analysis indicated that TRAM1 influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

Q15629

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids QLRRWREHSAFQAPAVKKK from the human protein were used as the immunogen for the TRAM1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the TRAM1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TRAM1 antibody is available for research use only.

Storage Conditions

After reconstitution, the TRAM1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TRAM1 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human SiHa cells with TRAM1 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= TRAM1 antibody.

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