UBA6 Antibody / UBE1L2

Modification of proteins with ubiquitin (UBB; MIM 191339) or ubiquitin-like proteins controls many signaling networks and requires a ubiquitin-activating enzyme (E1), a ubiquitin conjugating enzyme (E2), and a ubiquitin protein ligase (E3) . UBA6/UBE1L2 is an E1 enzyme that initiates the activation and conjugation of ubiquitin-like proteins.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

A0AVT1

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids I85-D1033) was used as the immunogen for the UBA6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the UBA6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This UBA6 antibody is available for research use only.

Storage Conditions

After reconstitution, the UBA6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the UBA6 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human ovarian cancer tissue with UBA6 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.