INF2 Antibody / Inverted formin-2

Inverted formin-2 is a protein that in humans is encoded by the INF2 gene. This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Immunoprecipitation: 2ug/500ug of lysate, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q27J81

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

E. coli-derived recombinant human protein (amino acids A7-Q674) was used as the immunogen for the INF2 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IF, FACS, IP, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the INF2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This INF2 antibody is available for research use only.

Storage Conditions

After reconstitution, the INF2 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the INF2 antibody should be determined by the researcher.

Location

Cytoplasmic, perinuclear

Image Legend

Immunofluorescent staining of FFPE human A549 cells with IFN2 antibody (green) and DAPI nuclear stain (blue) . HIER: steam section in pH6 citrate buffer for 20 min.