WNT10B Antibody

Protein Wnt-10b (formerly Wnt12[5]) is a protein that in humans is encoded by the WNT10B gene. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It may be involved in breast cancer, and its protein signaling is likely a molecular switch that governs adipogenesis. This protein is 96% identical to the mouse Wnt10b protein at the amino acid level. This gene is clustered with another family member, WNT1, in the chromosome 12q13 region.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells

UniProt

O00744

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids RLPHHSAILKRGFRE from the human protein were used as the immunogen for the WNT10B antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the WNT10B antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This WNT10B antibody is available for research use only.

Storage Conditions

After reconstitution, the WNT10B antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the WNT10B antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human PC-3 cells with WNT10B antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= WNT10B antibody.