NDUFS6 Antibody / NADH-ubiquinone oxidoreductase 13 kDa-A subunit

NADH dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial is an enzyme that in humans is encoded by the NDUFS6 gene. This gene encodes a subunit of the NADH:ubiquinone oxidoreductase (complex I), which is the first enzyme complex in the electron transport chain of mitochondria. This complex functions in the transfer of electrons from NADH to the respiratory chain. The subunit encoded by this gene is one of seven subunits in the iron-sulfur protein fraction. Mutations in this gene cause mitochondrial complex I deficiency, a disease that causes a wide variety of clinical disorders, including neonatal disease and adult-onset neurodegenerative disorders.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

O75380

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids M1-D88) was used as the immunogen for the NDUFS6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the NDUFS6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This NDUFS6 antibody is available for research use only.

Storage Conditions

After reconstitution, the NDUFS6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the NDUFS6 antibody should be determined by the researcher.

Location

Cytoplasmic (mitochondria)

Image Legend

Western blot testing of 1) rat brain, 2) rat heart, 3) rat kidney, 4) rat liver, 5) mouse brain, 6) mouse heart, 7) mouse kidney and 8) mouse liver tissue lysate with NDUFS6 antibody. Expected molecular weight ~13 kDa.