XIAP associated factor 1 Antibody / XAF1

XAF1, also called XIAP-associated factor 1, antagonizes the anti-caspase activity of XIAP and may be important in mediating apoptosis resistance in cancer cells. By genomic sequence analysis, the XAF1 gene contains 7 exons spanning 18 kb. Southern blot analysis suggested that XAF1 is a single-copy gene. The XAF1 gene is mapped on 17p13.1. Fluorescence microscopy demonstrated nuclear expression of XAF1, in contrast to the cytoplasmic expression of XIAP. Functional analysis indicated that XAF1 reverses the XIAP-mediated inhibition of CASP3 and its protection against apoptosis. Northern blot analysis detected wide expression of 3.9-, 4.5-, 6.0-, and 7.0-kb XAF1 transcripts, with highest levels in heart and ovary and lowest levels in brain and testis. Antisense experiments determined that loss of XAF1 expression enhances resistance to apoptosis. Alteration in XAF1 and XIAP RNA expression levels may lead to increased apoptotic resistance and proliferation due to unregulated XIAP function in cancer cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q6GPH4

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids Q197-S301) was used as the immunogen for the XIAP associated factor 1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the XIAP associated factor 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This XIAP associated factor 1 antibody is available for research use only.

Storage Conditions

After reconstitution, the XIAP associated factor 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the XIAP associated factor 1 antibody should be determined by the researcher.

Location

Cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human thyroid cancer tissue with XIAP associated factor 1 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.