Von Willebrand Factor Antibody / VWF

Von Willebrand factor (VWF) is a blood glycoprotein involved in hemostasis. It is mapped to 12p13.31. The VWF gene encodes von Willebrand factor (VWF), a large multimeric glycoprotein that plays a central role in the blood coagulation system, serving both as a major mediator of platelet-vessel wall interaction and platelet adhesion, and as a carrier for coagulation factor VIII. VWF released from endothelial cell Weibel-Palade bodies bound particularly avidly to the extracellular matrix. VWF deficiency or dysfunction (von Willebrand disease) leads to a bleeding tendency, which is most apparent in tissues having high blood flow shear in narrow vessels.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P04275

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids R1287-Q2770) was used as the immunogen for the von Willebrand Factor antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the von Willebrand Factor antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This von Willebrand Factor antibody is available for research use only.

Storage Conditions

After reconstitution, the von Willebrand Factor antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the von Willebrand Factor antibody should be determined by the researcher.

Image Legend

IHC staining of FFPE human tonsil tissue with von Willebrand Factor antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.