TRPV6 Antibody / CaT-L / CaT1

TRPV6 (Transient Receptor Potential Cation Channel Subfamily V Member 6), also known as ECAC2, CAT1 or CATL, a membrane calcium channel which is responsible for the first step in calcium absorption in the intestine. TRPV6 is a member of the Transient receptor potential (TRP) family of membrane proteins. Using FISH, Peng et al. (2000) mapped the TRPV6 gene to chromosome 7q33-q34. Peng et al. (2000) determined that TRPV6 mediates calcium uptake independent of sodium, chloride, and hydrogen; however, calcium influx was higher at alkaline rather than acidic pH. Functional analysis by Peng et al. (2001) indicated that TRPV5 mediates greater calcium uptake than TRPV6. They concluded that TRPV5 and TRPV6 are calcium-selective channels and share a common ancestral gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q9H1D0

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids A15-I765) was used as the immunogen for the TRPV6 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the TRPV6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TRPV6 antibody is available for research use only.

Storage Conditions

After reconstitution, the TRPV6 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TRPV6 antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human RT4 cells with TRPV6 antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= TRPV6 antibody.