TOMM20 Antibody

Mitochondrial import receptor subunit TOM20 homolog is a protein that in humans is encoded by the TOMM20 gene. Enables protein-transporting ATPase activity and unfolded protein binding activity. Involved in protein targeting to mitochondrion. Located in mitochondria-associated endoplasmic reticulum membrane and mitochondrial outer membrane.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Immunoprecipitation: 2ug/500ug of lysate, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q15388

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids D25-E145) was used as the immunogen for the TOMM20 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, IP, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the TOMM20 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This TOMM20 antibody is available for research use only.

Storage Conditions

After reconstitution, the TOMM20 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the TOMM20 antibody should be determined by the researcher.

Location

Cytoplasmic (mitochondria membrane)

Image Legend

IHC staining of FFPE human lung cancer tissue with TOMM20 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.