Ki-67 Antibody / MKI67

Ki-67 (Proliferation-related Ki-67 antigen), also known as MKI67 or KIA, is a protein that in humans is encoded by the MKI67 gene. From study of a panel of human-rodent somatic cell hybrids, it has been demonstrated that a gene involved in the expression of the MKI67 antigen is located on chromosome 10. By in situ hybridization, Fonatsch et al. (1991) regionalized the MKI67 gene to chromosome 10q25-qter. By FISH, Traut et al. (1998) mapped the mouse Mki67 gene to chromosome 7F3-F5. Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67 leads to inhibition of ribosomal RNA synthesis.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

P46013

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids R5-Q2358) was used as the immunogen for the Ki-67 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Ki-67 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Ki-67 antibody is available for research use only.

Storage Conditions

After reconstitution, the Ki-67 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Ki-67 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Ki-67 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.