CCDC115 Antibody

Coiled-coil domain containing 115 is a protein that in humans is encoded by the CCDC115 gene. The protein encoded by this gene has been observed to localize to the endoplasmic reticulum (ER) -Golgi intermediate compartment (ERGIC) and coat protein complex I (COPI) vesicles in some human cells. The encoded protein shares some homology with the yeast V-ATPase assembly factor Vma22p, and the orthologous protein in mouse promotes cell proliferation and suppresses cell death. Defects in this gene are a cause of congenital disorder of glycosylation, type IIo in humans.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q96NT0

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant human protein (amino acids M1-A180) was used as the immunogen for the CCDC115 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, IF, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the CCDC115 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This CCDC115 antibody is available for research use only.

Storage Conditions

After reconstitution, the CCDC115 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the CCDC115 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human ovarian cancer tissue with CCDC115 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.