BRDT Antibody / Bromodomain testis-specific protein

Bromodomain testis-specific protein is a protein that in humans is encoded by the BRDT gene. BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Flow cytometry: 1-3ug/million cells, Direct ELISA: 0.1-0.5 µg/mL

UniProt

Q58F21

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human BRDT protein (amino acids Q795-D947) was used as the immunogen for the BRDT antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, FACS, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the BRDT antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This BRDT antibody is available for research use only.

Storage Conditions

After reconstitution, the BRDT antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the BRDT antibody should be determined by the researcher.

Image Legend

Flow cytometry testing of human HeLa cells with BRDT antibody at 1ug/million cells (blocked with goat sera) ; Red=cells alone, Green=isotype control, Blue= BRDT antibody.