Alpha Fetoprotein Antibody / AFP
AFP, also called Alpha-fetoprotein, is a protein that in humans is encoded by the AFP gene. It is mapped to 4q13.3. The level of AFP in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. In rodents AFP binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. Moreover, it has an important role as a diagnostic marker, especially in certain tumors and liver diseases of childhood. AFP is also used to test the potential usefulness of plasma alpha fetoprotein determination as a diagnostic marker in biliary atresia, hepatitis, and yolk sac derived tumours.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Direct ELISA: 0.1-0.5 µg/mL
UniProt
P02771
Host
Rabbit
Reactivity
Human
Immunogen
Recombinant human protein (amino acids R19-L258) was used as the immunogen for the Alpha Fetoprotein antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
WB, Direct ELISA
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
Limitations
This Alpha Fetoprotein antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the Alpha Fetoprotein antibody should be determined by the researcher.
Image Legend
Western blot testing of human 1) HepG2 and 2) HCCP cell lysate with Alpha Fetoprotein antibody. Predicted molecular weight: ~70 kDa.
Frequently Asked Questions
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