FLOT2 Antibody / Flotillin 2
FLOT2 (Flotillin 2), also known as ESA1 or M17S1, is a protein that in humans is encoded by the FLOT2 gene. Schroeder et al. (1991) isolated a cDNA for an epidermal surface antigen believed to be involved in epidermal cell adhesion. By analysis of a somatic cell hybrid panel and in situ hybridization using the ESA cDNA, the gene was mapped to 17q11-q12 in the region containing the NF1 gene. Bickel et al. (1997) found that mouse Flot2 consistently copurifies with Flot1 and with caveolin-1 in the purification of caveolin-rich membranes. Using a quantitative proteomic analysis of cultured mouse neuronal stem cells, Li et al. (2012) found that palmitoylation and oligomerization of flotillin-2 was abolished in homozygous Dhhc5 mutant neuronal stem cells. The absolute amount of flotillin-2 was not changed in Dhhc5 mutant neurons.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Immunofluorescence: 5 µg/mL
UniProt
Q14254
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein (amino acids K169-K344) was used as the immunogen for the FLOT2 antibody.
Clonality
Monoclonal
Isotype
IgG2b
Clone
4D8A3
Applications
WB, IHC-P, IF
Purity
Antigen affinity purified
Format
Antigen affinity purified
Buffer
Lyophilized from 1X PBS with 2% Trehalose
Reconstitution
Limitations
This FLOT2 antibody is available for research use only.
Storage Conditions
Formulation
0.5 mg/mL if reconstituted with 0.2ml sterile DI water
Applications Notes
Optimal dilution of the FLOT2 antibody should be determined by the researcher.
Location
Cytoplasmic, membrane
Image Legend
IHC staining of FFPE human placental tissue with FLOT2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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