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Protein arginine N-methyltransferase 5 Antibody / PRMT5

Protein arginine N-methyltransferase 5is an enzyme that in humans is encoded by the PRMT5 gene. It is mapped to 14q11.2. This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation, and the assembly of small nuclear ribonucleoproteins. A pseudogene of this gene has been defined on chromosome 4. Alternative splicing results in multiple transcript variants encoding different isoforms.

Product Specifications

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 2-5 µg/mL, Direct ELISA: 0.1-0.5 µg/mL

UniProt

O14744

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant human protein (amino acids S16-D355) was used as the immunogen for the Protein arginine N-methyltransferase 5 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

WB, IHC-P, Direct ELISA

Purity

Antigen affinity purified

Format

Antigen affinity purified

Buffer

Lyophilized from 1X PBS with 2% Trehalose

Reconstitution

After reconstitution, the Protein arginine N-methyltransferase 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Limitations

This Protein arginine N-methyltransferase 5 antibody is available for research use only.

Storage Conditions

After reconstitution, the Protein arginine N-methyltransferase 5 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Product Datasheet

https://www.nsjbio.com/tds-pdf/protein-arginine-n-methyltransferase-5-antibody-prmt5-rq7102

Formulation

0.5 mg/mL if reconstituted with 0.2ml sterile DI water

Applications Notes

Optimal dilution of the Protein arginine N-methyltransferase 5 antibody should be determined by the researcher.

CAS Number

9007-83-4

Location

Cytoplasmic, nuclear

Image Legend

IHC staining of FFPE human laryngeal squamous cell carcinoma tissue with Protein arginine N-methyltransferase 5 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
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