Thidiazuron (TDZ)

Thidiazuron (TDZ) Powder is a plant growth regulator and a synthetic diphenylurea cytokinin widely used in plant tissue culture applications to promote shoot regeneration, organogenesis, and somatic embryogenesis.

Product Specifications

CAS Number

51707-55-2

Synonyms

1-Phenyl-3-(1,2,3-thiadiazol-5-yl)urea; TDZ

HS Code

2934.99

Applications

Used alone or in combination with other hormones for efficient multiplication of in vitro plants. TDZ can be used in culture media to promote seed germination Used in agricultural and home gardening. TDZ is often used in combination with auxin (IAA) in culture media to achieve a balance between shoot and root development.

Assay Protocol

Follow the given steps to prepare the tissue culture media using PCT’s TDZ Powder: Prepare liquid tissue culture media using PCT’s MS or DKW media, or any other alternative media based on your plant. Add sugar based on the quantity of media you’re making (generally for 1-liter media 30 grams of sugar is added), 1 ml/L PPM, and 3-4 grams per liter of gellan gum or 6-8 grams per liter of agar. Then, add the desired quantity of the plant hormone from the prepared NAA stock solution. Autoclave the medium for 20 minutes at 15 psi and 121°C. Your media is ready!

Color

White to Off-White Powder or Crystals

Solubility

Soluble in DMSO, KOH, MeOH

Molecular Formula

C9H8N4OS

Features & Benefits

Easy to use Effective in stimulating shoot multiplication from explants (tissue samples) in micropropagation Used to supplement tissue culture media, such as MS media. Help overcome apical dominance Crucial for the micropropagation of some plants.