M7GpppAmpG (ammonium)

M7GpppAmpG ammonium (m7G (5') ppp (5') (2'OMeA) pG ammonium) is a trinucleotide 5′ end cap analog. m7GpppAmpG ammonium binds to eIF4E with a KD value of 45.6 nM. m7GpppAmpG ammonium caps RNA with a capping efficiency of 90%. m7GpppAmpG ammonium enhances mRNA stability and translation efficiency. m7GpppAmpG ammonium is used in mRNA therapeutic research[1][2][3].

Product Specifications

Product Name Alternative

M7G (5') ppp (5') (2'OMeA) pG ammonium

UNSPSC

12352005

Target

DNA/RNA Synthesis; Eukaryotic Initiation Factor (eIF)

Type

Oligonucleotides

Related Pathways

Cell Cycle/DNA Damage

Applications

Cancer-programmed cell death

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/m7gpppampg-ammonium.html

Concentration

100 mM * 10 μL in Water

Purity

99.62

Solubility

10 mM in DMSO

Smiles

O=P(O)(OC[C@@H]1[C@@H](OP(O)(OC[C@H]2O[C@@H](N3C(NC(N)=NC4=O)=C4N=C3)[C@H](O)[C@@H]2O)=O)[C@@H](OC)[C@H](N5C=NC6=C(N)N=CN=C65)O1)OP(OP(OC[C@H]7O[C@@H](N8C(NC(N)=N9)=C([N+](C)=C8)C9=O)[C@H](O)[C@@H]7O)([O])=O)(O)=O.[xNH3]

Molecular Formula

C32H43N15O24P4.xNH3

References & Citations

[1]Sikorski PJ, Warminski M, Kubacka D, et al. The identity and methylation status of the first transcribed nucleotide in eukaryotic mRNA 5' cap modulates protein expression in living cells. Nucleic Acids Res. 2020;48 (4) :1607-1626. |[2]Zhang H, et al. GPT-Driven Generation and Biological Activity Evaluation of Novel mRNA Trinucleotide Cap1 Analogs for mRNA Vaccine or Immunotherapy. Journal of Materials Chemistry B, 2025.|[3]Raab M, et al. Rescue of p53 functions by in vitro-transcribed mRNA impedes the growth of high-grade serous ovarian cancer. Cancer Commun (Lond) . 2024 Jan;44 (1) :101-126.