Human IL-3 Protein, premium grade

Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The human IL-3 reported to be a monomer, as it is known, contains 133 amino acids residues which is a single non-glycosylated polypeptide. Specifically, human and murine IL-3 share low homology and it does not show activity on murine cells.

Product Specifications

Background

Specifications

This protein carries no "tag". The protein has a calculated MW of 15.1 kDa. The protein migrates as 14 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) .

Accession Number

P08700-1

Expression Region

Ala 20 - Phe 152

Host

E. coli

Target

IL-3

Conjugation

Unconjugated

Tag

Native

Source

Human

Stability

-20°C to -70°C for 24 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

95%

Bioactivity

Immobilized Human IL-3 Protein, premium grade (Cat. No. IL3-H5115) at 10 μg/mL (100 μL/well) can bind Biotinylated Human IL-3 R alpha, Fc,Avitag (Cat. No. ILA-H82F3) with a linear range of 1.2-40 ng/mL (QC tested).