Anti-MEK2 (Phospho-Thr394) Antibody

Rabbit Polyclonal Antibody specific to MEK2 (Phospho-Thr394)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-MEK2 (Phospho-Thr394) Rabbit Polyclonal Antibody

Gene Name

MAP2K2

Gene ID

601263

NCBI Gene ID

UniProt

P36507

Cellular Locus

Cytoplasm, Membrane

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of threonine (P-G-T(p)-P-T) derived from human MEK2 and conjugated to KLH.

Target Antigen

Dual specificity mitogen-activated protein kinase kinase 2

Target

MEK2 (Phospho-Thr394)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases (By similarity). Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (PubMed:29433126). {UniProtKB:Q63932, PubMed:29433126}.

Additionnal Information

Western blotting: use at dilution of 1:500- 1:1,000. A band of ~44kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human MEK2 only when phosphorylated at threonine 394.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

MEK2 is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase plays an important role in mitogen growth factor signal transduction. It phosphorylates and activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax.