Human Integrin alpha 11 beta 1 (ITGA11&ITGB1) Heterodimer Protein, His Tag&Tag Free

Integrin alpha 11 beta 1 is a receptor for collagen. The alpha11 cDNA encodes a mature protein with a large 1120-residue extracellular domain that contains an I-domain of 207 residues and is linked by a transmembrane domain to a short cytoplasmic domain of 24 amino acids. The deduced alpha11 protein shows the typical structural features of integrin alpha-subunits and is similar to a distinct group of alpha-subunits from collagen-binding integrins.

Product Specifications

Background

Specifications

Human ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA11 and ITGB1, has a calculated MW of 131.6 kDa (ITGA11) and 82.7 kDa (ITGB1) . Subunit ITGA11 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The protein migrates as > 200 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q9UKX5-1 (L524R) (ITGA11) & P05556-1 (ITGB1)

Expression Region

Phe 23 - Pro 1141 (ITGA11) & Gln 21-Asp 728 (ITGB1)

Host

HEK293

Target

Integrin alpha 11 beta 1

Conjugation

Unconjugated

Tag

C-10xHis | Native

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA11&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W3) with a linear range of 0.078-1.25 μg/mL (QC tested).