V5 tag, AlpHcAbs® Rabbit

Anti-V5 tag, AlpHcAbs® Rabbit antibody is designed for detecting V5 tag fusion protein specifically. Anti-V5 tag, AlpHcAbs® Rabbit antibody is monovalent, recombinant single domain antibody fused to rabbit IgG Fc. Based on western blot and ELISA, Anti-V5 tag, AlpHcAbs® Rabbit antibody reacts with the V5 tag sequence (GKPIPNPLLGLDST) selectively, no reactivity with other proteins. High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production.

Product Specifications

Host

Alpaca

Species Reactivity

Universal

Immunogen

V5 tag fusion protein

Conjugation

Non-conjugated

Type

Alpaca monoclonal Antibody

Applications

WB, ICC/IF, ELISA, IP, Flow Cyt

Concentration

1 mg/mL

Dilution

WB: 1:10,000-1:50,000 ;ELISA: 1:10,000-1:50,000 ;ICC/IF: 1:200-1:1000 ;IP: 1-2ug/sample ;Flow Cyt:1µg for 106 cells ;Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.