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Recombinant Napsin A Antibody / Rabbit Monoclonal

Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

Product Specifications

UniProt

O96009

Reactivity

Human

Immunogen

A recombinant fragment from the human protein (amino acids 189-299) was used as the immunogen for the recombinant Napsin A antibody.

Clonality

Recombinant

Clone

NAPSA/1865R

Conjugation

Unconjugated

Field of Research

Epigenetics

Purification

Protein A affinity chromatography

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Western blot: 2-4ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The stated application concentrations are suggested starting points. Titration of the recombinant Napsin A antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

IHC-P, WB

Host or Source

Rabbit

Preservative

0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

Isotype

Rabbit IgG, kappa

Available Sizes

Frequently Asked Questions

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