ErbB2 Antibody / HER2

This antibody recognizes a protein of 185kDa, which is identified as HER2 / ErbB2. Its epitope is localized in the extracellular domain. ErbB2 is a member of the EGFR family. This antibody is specific and shows minimal cross-reaction with other members of the EGFR-family. Receptors of this family are located on the plasma membrane and consist of an extracellular ligand-binding domain that is connected to a large intracellular domain by a single transmembrane sequence. ErbB2 protein is over-expressed in a variety of carcinomas especially those of breast and ovary.

Product Specifications

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this ErbB2 antibody. (1)

Clonality

Monoclonal

Clone

HRB2/258

Conjugation

Unconjugated

Field of Research

Signal Transduction

Purification

Protein G purified

Dilution

ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Variations in protocols, secondaries and substrates may require the antibody to be titered for optimal performance.1. This ErbB2 antibody binds to the extracellular/cell surface region of the protein.