7-Ketocholesterol Antibody
Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG2b
Product Specifications
Product Name Alternative
7-Ketocholesterol, 7-Keto Cholesterol, 7-Oxocholesterol, Δ5-Cholesterol-3β-ol-7-one, 5-Cholesten-3β-ol-7-one, Cholest-5-en-3β-ol-7-one, 3β-Hydroxy-5-cholesten-7-one, 3-Hydroxy-5-cholestene-7-one, SC-4722
Reactivity
All
Immunogen
Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH) .
Target
7-Ketocholesterol
Clonality
Monoclonal
Clone
70
Conjugation
Unconjugated
Field of Research
Cancer Biology, Cell Biology, Metabolism Research
Purification
Protein G Purified
Concentration
1 mg/ml
Dilution
WB (1:1000) ; ICC/IF (1:50) ; ELISA (1:1000)
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
A 1:1000 dilution was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
Tested Applications
ELISA, ICC, IF, WB
Host or Source
Mouse
Preservative
PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
Isotype
IgG2b
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