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7-Ketocholesterol Antibody

Mouse Anti-7-Ketocholesterol (7-KC) Monoclonal IgG2b

Product Specifications

Product Name Alternative

7-Ketocholesterol, 7-Keto Cholesterol, 7-Oxocholesterol, Δ5-Cholesterol-3β-ol-7-one, 5-Cholesten-3β-ol-7-one, Cholest-5-en-3β-ol-7-one, 3β-Hydroxy-5-cholesten-7-one, 3-Hydroxy-5-cholestene-7-one, SC-4722

Reactivity

All

Immunogen

Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH) .

Target

7-Ketocholesterol

Clonality

Monoclonal

Clone

70

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cell Biology, Metabolism Research

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000) ; ICC/IF (1:50) ; ELISA (1:1000)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

A 1:1000 dilution was sufficient for detection of 7-Ketocholesterol in 2 μg of 7-ketocholesterol conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.

Tested Applications

ELISA, ICC, IF, WB

Host or Source

Mouse

Preservative

PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgG2b

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